The Buckinghamshire Railway was a railway company in Buckinghamshire and Oxfordshire, England that constructed railway lines connecting Bletchley, Banbury and Oxford. Part of the route is still in use today as the Oxford to Bicester Line.
The origins of the Buckinghamshire Railway can be traced back to the gauge and territorial wars which took place in Buckinghamshire in the 1840s. The London and Birmingham Railway (L&B) had opened a standard gauge line from Euston to Tring on 16 October 1837, extending to Birmingham in September 1838. In June of that year, the Great Western Railway (GWR) opened the first part of its broad gauge line from Paddington to Bristol and a further branch from Didcot to Oxford had been opened by June 1844. The GWR subsequently looked to expand beyond Oxford by depositing bills for the Oxford, Worcester and Wolverhampton Railway and the Oxford and Rugby Railway which would take it into the heart of the Midlands.