The Botanischer Garten Jena (4.5 hectares) is the second oldest botanical garden in Germany, maintained by the University of Jena and located at Fürstengraben 26, Jena, Thuringia, Germany. It is open daily; an admission fee is charged.
The garden was first established in 1586 as a hortus medicus, six years after the establishment of the Botanical Garden in Leipzig in 1580. In 1630 it was rearranged and expanded significantly by Professor Werner Rolfinck who had previously studied at the Orto botanico di Padova (founded 1545). In 1640 a second section (1.3 hectares in area, north of the city walls) was donated, and a catalog from 1659 documents over 1300 plants in the two gardens. In 1662 the original garden was expanded, with the first heated greenhouse added in 1674, at which time the garden first began to maintain a collection of tropical plants.
In 1770 the garden introduced Linnean taxonomy, and in 1776 Goethe began his association with the garden and helped organize the Jena Institute of botany; over subsequent decades he often studied botany and wrote poems in the garden. Although the garden area was only 1.3 hectares at this time, purchases recorded in 1794 include Buxus sempervirens, Juniperus sabina, Periploca graeca, Sambucus racemosa, and Thuja occidentalis. Several years later, additional purchases included amaryllis, cacti, succulent Euphorbia, Pelargonium, and Zantedeschia. The garden's first published catalog issued in 1795.