Alsike Church (Swedish: Alsike kyrka) is a Lutheran church in the Archdiocese of Uppsala in Uppsala County, Sweden. It lies in Knivsta Municipality, a suburb to Stockholm.
Alsike Church stands by a formerly important sea-route linking Uppsala, Sigtuna and , in an area characterised by its old cultural landscape. Although the oldest parts of the presently visible church date from the 13th century, it was probably pre-dated by an earlier wooden church. The church is constructed largely in brick, a material not usually used in medieval churches in Uppland (fieldstone was often the material of choice). The use of brick may be explained by the vicinity to the more costly churches in Sigtuna or Sko Abbey (near Skokloster Castle). The main part of the church was probably built circa 1250-1350. The tower and the church porch date from the 15th century, and during the same century the church was also equipped with frescoed vaults; due to later damage these have been rebuilt and only fragments of the frescos still remain.
The church was rebuilt during the mid-19th century. Two transepts were added, and the exterior whitewashed. Inside the church was equipped with a new pulpit and new pews. In 1910-11, the church was redecorated internally with frescos in Art Nouveau style by architect Sigurd Curman. During repairs in 1947 a runestone was discovered in the church; it is displayed at the church entrance.