The 2016 NCAA Men's Division I Basketball Tournament involved 68 teams playing in a single-elimination tournament to determine the national champion of men's NCAA Division I college basketball. The 78th edition of the tournament began on March 15, 2016, and concluded with the championship game on April 4 at NRG Stadium in Houston, Texas.

Upsets were the story of the First Round of the Tournament; No. 15 seed Middle Tennessee upset No. 2 seed Michigan State in the biggest upset, just the eighth ever win for a No. 15 seed over a No. 2. At least one 9, 10, 11, 12, 13, 14, and 15 seed won a first-round game for the third time ever and the first time since 2013. Also, every seed except a 16 won at least one game in the first round.

In the Final Four, Villanova defeated Oklahoma, while North Carolina defeated Syracuse. Villanova then defeated North Carolina to win the championship on a three-point buzzer beater by Kris Jenkins. Pundits called the game one of the best in Tournament history, going on to say this was one of the most competitive finals ever.

Previously, the Round of 64 was known as the Second Round since the 2011 edition, but it was reverted to the moniker First Round for this coming tournament. The First Four was previously named the First Round.

First Four

First and Second Rounds

Regional Semifinals and Finals (Sweet Sixteen and Elite Eight)

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