The 2010 Premier League Knock-Out Cup was contested throughout the Premier League season. The reigning champions were the Kings Lynn Stars. The 2010 champions were the Newcastle Diamonds after beating the Edinburgh Monarchs 91-88 over a two legged final.
The draw for the 2010 Premier League KOC was taking at the 2009 AGM. 12 teams were drawn against each other, with 2 teams receiving 'byes' into the Quarter Finals. These two teams that received a bye were the Redcar Bears and the Somerset Rebels.