2000 du Maurier Open – Women's Singles
| Women's Singles | |
|---|---|
| 2000 du Maurier Open | |
| Champion |
 Martina Hingis
|
| Runner-up |
 Serena Williams
|
| Final score | 0–6, 6–3, 3–0 retired |
The 2000 du Maurier Open – Women's Singles was the women's singles event of the one hundred and eleventh edition of the Canadian Open; a WTA Tier I tournament and the most prestigious women's tennis tournament held in Canada. Martina Hingis was the defending champion, and won in the final 0–6, 6–3, 3–0, after Serena Williams retired due to a left foot injury.
The top eight seeds receive a bye into the second round.
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