2000 Rome Masters – Women's Singles
| Women's Singles | |
|---|---|
| 2000 Rome Masters | |
| Champion |
 Monica Seles
|
| Runner-up |
 Amélie Mauresmo
|
| Final score | 6–2, 7–6(7–4) |
Venus Williams was the defending champion, but lost in third round to Jelena Dokic.
Monica Seles won the title by defeating Amélie Mauresmo 6–2, 7–6(7–4) in the final. It was the 3rd title of the year for Seles and the 47th of her career.
The first eight seeds received a bye into the second round.
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