| Season | 2000–01 |
|---|---|
| Champions | Moreirense FC |
| Promoted |
UD Oliveirense Moreirense FC Portimonense SC |
| Relegated | 12 teams |
|
2001–02 →
|
|
The 2000–01 Segunda Divisão season was the 67th season of the competition and the 51st season of recognised third-tier football in Portugal.
The league was contested by 59 teams in 3 divisions with UD Oliveirense, Moreirense FC and Portimonense SC winning the respective divisional competitions and gaining promotion to the Liga de Honra. The overall championship was won by Moreirense FC.