2000–01 Biathlon World Cup

The 2000–01 Biathlon World Cup was a multi-race tournament over a season of biathlon, organised by the International Biathlon Union. The season started on 30 November 2000 in Antholz-Anterselva, Italy, and ended on 18 March 2001 in Holmenkollen, Norway. It was the twenty-fourth season of the Biathlon World Cup.

Below is the IBU World Cup calendar for the 2000–01 season.

Following notable biathletes announced their retirement during or after the 2000–01 season: