The 1968–69 Regionalliga was the sixth season of the Regionalliga, the second tier of the German football league system. The league operated in five regional divisions, Berlin, North, South, Southwest and West. The five league champions and all five runners-up, at the end of the season, entered a promotion play-off to determine the two clubs to move up to the Bundesliga for the next season. The two promotion spots went to the Regionalliga West champions and runners-up Rot-Weiß Oberhausen and Rot-Weiß Essen.
The 1968–69 season saw two new clubs in the league, Heider SV and TuS Celle, both promoted from the Amateurliga, while no club had been relegated from the Bundesliga to the league.
The 1968–69 season saw three new clubs in the league, Meteor 06 Berlin, VfL Nord Berlin and SC Staaken, all three promoted from the Amateurliga, while no club had been relegated from the Bundesliga to the league. For the following season the league was reduced from 16 to 14 clubs.
The 1968–69 season saw two new clubs in the league, Bonner SC and Eintracht Duisburg, both promoted from the Amateurliga, while no club had been relegated from the Bundesliga to the league.
The 1968–69 season saw three new clubs in the league, FV Speyer and FC Landsweiler, both promoted from the Amateurliga, while Borussia Neunkirchen had been relegated from the Bundesliga to the league.