The 1959 Major League Baseball All-Star Game was the 27th playing of the midsummer classic between the all-stars of the American League (AL) and National League (NL), the two leagues composing Major League Baseball. The game was played on August 3, 1959, at the Los Angeles Memorial Coliseum in Los Angeles, California, home of the Los Angeles Dodgers of the NL. The game resulted in a 5–3 victory for the American League. This was the second of two All-Star Games played in 1959, the first game having been played on July 7 in Pittsburgh, Pennsylvania.

This is one of only two All-Star Games to be played outside of the month of July, the other being in 1981.

Players in italics have since been inducted into the National Baseball Hall of Fame.

Umpires: Charlie Berry, Home Plate (AL); Bill Jackowski, First Base (NL); Bill Summers, Second Base (AL); Tony Venzon, Third Base (NL); Hank Soar, Left Field (AL); Ken Burkhart, Right Field (NL)