Ōtsu-juku (大津宿 Ōtsu-juku?) was the last of the sixty-nine stations of the Nakasendō, as well as the last of the fifty-three stations of the Tōkaidō. It was 14 km (9 mi) from the previous post town, Kusatsu-juku, and was located in Ōmi Province. It is currently located in the present-day city of Ōtsu, Shiga Prefecture, Japan.
Ōtsu-juku was a rather large post town, with approximately 350 homes, two honjin, a secondary honjin and 71 inns for other travelers.
Coordinates: 35°00′21.5″N 135°51′41.1″E / 35.005972°N 135.861417°E