Ōi-juku (大井宿 Ōi-juku?) was the forty-sixth of the sixty-nine stations of the Nakasendō. It is located in the present-day city of Ena, Gifu Prefecture, Japan. From Ōi-juku to the next post town, Ōkute-juku, there are 13 hills to pass over. Travellers using the Shitakaidō (下街道) often used Ōi-juku, too, as they travelled on to Makiganetsui (槙ヶ根追) afterwards.
Much of Ōi-juku was laid out using right angles, giving it a very ordered appearance. The other parts of the post town were named Yoko-chō, Hon-machi, Tate-machi, Chaya-chō and Hashiba. It was near the banks of the Agi River.
Coordinates: 35°27′20.1″N 137°24′53.8″E / 35.455583°N 137.414944°E