The Vasse-Wonnerup Estuary is an estuary in the South West region of Western Australia close to the town of Busselton. The estuary is listed with DIWA. It was also recognised as a wetland of international importance under the Ramsar Convention on 7 June 1990 when an area of 1115 ha was designated Ramsar Site 484 as an important dry-season habitat for waterbirds. It is also the main part of the 2038 ha Busselton Wetlands Important Bird Area.
The estuary is wave dominated and has been severely modified from its natural state. The site of the Vasse and Wonnerup Floodgates that regulated the flow of water in the estuary from about 1907 inadvertently created the freshwater wetland, were listed on the Western Australian Register of Heritage Places in 2005. The estuary covers a total surface area of 17.8 square kilometres (7 sq mi) with the central basin having an area of 7.5 square kilometres (3 sq mi) In winter, wide areas of open water are fringed by samphire and rushes. Paperbark woodland occurs behind the samphire belt, with eucalypt woodland on higher ground.
The catchment of the estuary is 52% cleared and is used primarily for crops and pastures but also for plantations. The waters are susceptible to pollution from run-off as houses in Busselton have been built adjacent to the estuary. The Abba River, Ludlow River, Sabina River and Vasse River all discharge into the estuary.
The catchment covers a total area of 961 square kilometres (371 sq mi) and is part of the Geographe Bay catchment. The coastal plain area is composed of sandy and duplex soils that are easily water-logged. An extensive drainage network has been constructed to protect Busselton and Wonnerup from flooding and to create agricultural land.