The Women's United States Squash Open 2016 is the women's edition of the 2016 United States Open (squash), which is a WSA World Series event (Prize money: $150 000). The event took place at the Daskalakis Athletic Center in Philadelphia, Pennsylvania in the United States from the 8th of October to the 15th of October. Camille Serme won her first US Open trophy, beating Nour El Sherbini in the final.

For 2016, the prize purse was $150,000. The prize money and points breakdown is as follows: