Urbano Barberini (1664 – 27 September 1722) was an Italian nobleman of the House of Barberini, third hereditary Prince of Palestrina and last legitimate male heir of the Barberini line from Pope Urban VIII.
Urbano Barberini was the fourth child of the marriage, of Maffeo Barberini and Olimpia Giustiniani, which had reconciled the Barberini with the papacy after the Wars of Castro and falling out with Pope Innocent X.
He was a brother of Cardinal Francesco Barberini (Junior) and a cousin of Rinaldo d'Este, Duke of Modena whose mother, Lucrezia Barberini, was Urbano's aunt.
At his father's death he inherited the Barberini comune of Palestrina and became the 3rd Barberini Prince of Palestrina. Like his father, he was made a Knight of the Order of the Golden Fleece (in 1687).
The legitimate male Barberini line became extinct at Urbano's death in 1722 and the Barberini name and title's were transferred to Giulio Cesare and the House of Colonna di Sciarra.
Barberini was married three times.
In 1691 he married Cornelia Zeno Ottoboni (sister of Cardinal Pietro Ottoboni). Her last will and testament gives some idea of the wealth the Barberini had amassed under Urbano's grandfather, Taddeo Barberini and his brothers who had all benefited from their uncle Pope Urban VIII's famous nepotism.