Trans-splicing

Trans-splicing is a special form of RNA processing in eukaryotes where exons from two different primary RNA transcripts are joined end to end and ligated.

Whereas "normal" (cis-)splicing processes a single molecule, trans-splicing generates a single RNA transcript from multiple separate pre-mRNAs. This phenomenon can be exploited for molecular therapy to address mutated gene products.

Trans-splicing can be the mechanism behind certain oncogenic fusion transcripts.

Trans-splicing is used by certain microbial organisms, notably protozoa of the Kinetoplastae class to express genes. In these organisms, a capped splice leader RNA is transcribed; simultaneously, genes are transcribed in long polycistrons. The capped splice leader is trans-spliced onto each gene to generate monocistronic capped and polyadenylated transcripts.

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