Toluylene red

Neutral red

Solid Neutral Red Neutral Red Aqueous Solution
Names
IUPAC name 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride
Other names toluylene red
Identifiers
CAS Number	553-24-2 Y
3D model (Jmol)	Interactive image
ChEBI	CHEBI:86370 N
ChemSpider	10634 Y
ECHA InfoCard	100.008.215
InChI InChI=1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H Y Key: PGSADBUBUOPOJS-UHFFFAOYSA-N Y InChI=1/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H Key: PGSADBUBUOPOJS-UHFFFAOYAB
SMILES Cl.n1c3c(nc2c1cc(c(c2)N)C)cc(N(C)C)cc3
Properties
Chemical formula	C15H17ClN4
Molar mass	288.78 g/mol
Melting point	290 °C (554 °F; 563 K)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N  (what is YN ?)
Infobox references

Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood. Can be used for staining Golgi apparatus in cells and Nissl granules in neurons.

In microbiology, it is used in the MacConkey agar to differentiate bacteria for lactose fermentation.

Neutral red can be used as a vital stain. Live cells incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus, loss of neutral red uptake corresponds to loss of cell viability. It is also used to stain cell cultures for plate titration of viruses.

Neutral red is added to some growth media for bacterial and cell cultures. It usually is available as a chloride salt.

Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0.