Thomas Crooke (c.1545–1598) was a sixteenth-century English clergyman, who was noted for his strongly Calvinist views. He was the father of the lawyer and politician Sir Thomas Crooke, 1st Baronet, founder of the town of Baltimore, County Cork, and of Helkiah Crooke, physician to King James I.
He was born at Cransley in Northamptonshire. From his will we know that he had several siblings and that his father after his mother's death remarried a Mrs. Joyner, who was still alive in 1595. His son Thomas in his will of 1629 left a legacy to "my good old Aunt Hudson", who was probably his father's sister; she was still alive in 1635. Sir George Croke, one of the High Court judges who heard the Case of Ship Money, is sometimes referred to as his cousin, but the exact connection between them is unclear.
He went to school in Stamford and matriculated at Trinity College, Cambridge in 1560, becoming scholar in 1562, Bachelor of Arts 1563 and Master of Arts 1566. He was ordained in 1568 in Norwich and presented to the living of Great Waldingfield, Suffolk in 1571. He graduated Doctor of Theology from Pembroke College, Cambridge in 1578.