Theodote (c. 780 – after 797) was the second Empress consort of Constantine VI of the Byzantine Empire. She was a member of a distinguished family of Constantinople and her brother Sergios was mentioned as an hypatos. Their mother Anna was a sister of Theoktiste and her brother Plato. Theodote was thus a maternal first cousin of Theodore the Studite, son of Theoktiste.
By 794, Theodote served as a lady-in-waiting (koubikoularia) of Irene. Irene was the widow of Leo IV the Khazar and mother of his heir Constantine VI. Irene had served as regent from 780 to 790 and still held the title of Empress.
Constantine was married to Maria of Amnia and the imperial couple had two daughters, Euphrosyne and a younger Irene. However, according to the chronicle of Theophanes the Confessor, Constantine had turned against his first wife at some point. Theophanes attributed the deterioration of the marriage to the machinations of Irene, but the lack of a male heir following six years of marriage could also be one of the reasons. Whatever the case, Constantine took Theodote as his royal mistress.
In January, 795, Constantine divorced Maria. Maria and both of their daughters were sent to a convent in the island of Prinkipo. In August, 795, Theodote was officially betrothed to the Emperor and proclaimed an Augusta, a title Maria had never been granted. In September, 795, Theodote and Constantine were married at the palace named after St. Mamas. Theodote had become the Empress consort within eight months of the removal of her predecessor.