Stephanie of Hohenzollern-Sigmaringen (Stephanie Josepha Friederike Wilhelmine Antonia; Portuguese: Estefânia; 15 July 1837 – 17 July 1859) was the Queen consort of King Peter V of Portugal.

Born in Krauchenwies, Sigmaringen, in 1837, Stephanie was the eldest daughter of Karl Anton, Prince of Hohenzollern, head of the House of Hohenzollern-Sigmaringen, and his wife Princess Josephine of Baden. Her maternal grandparents were Karl, Grand Duke of Baden, and Stéphanie de Beauharnais.

She was also a younger sister of Leopold, Prince of Hohenzollern, older sister of King Carol I of Romania, and aunt of King Albert I of Belgium.

Stephanie married King Peter V of Portugal by proxy on 29 April 1858 at St. Hedwig's Cathedral in Berlin, where her eldest brother Leopold stood in for the groom. She was then married in person on 18 May 1858 at the Church of St. Dominic in Lisbon. Both bride and groom were a few months short of their twenty-first birthdays. Stephanie was received with much luxury and wrote home that the Portuguese understood luxury better than dignity. However she fell ill with diphtheria and died only a year later in Lisbon at the age of 22. During her short period as queen, she made herself a good name from the foundation of hospitals.

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