The Second Holder Ministry was the 42nd Ministry of the Government of South Australia, led by Frederick Holder. It commenced on 8 December 1899, following the defeat of the seven-day Solomon Ministry. It was succeeded by the Jenkins Ministry on 15 May 1901, following Holder's retirement from state politics to enter federal politics, and his replacement as leader of the liberal faction in parliament by his Chief Secretary, John Jenkins.
Premier
Treasurer
Minister of Industry
Chief Secretary
Commissioner of Crown Lands
Commissioner of Public Works
Minister of Education and Agriculture