Lt. Col. Samuel Mathews (1630–1660), Commonwealth Governor of Virginia, of Warwick County in the English Colony of Virginia, was a member of the House of Burgesses, the Governor's Council, and served as Commonwealth Governor of Virginia from 1656 until he died in office in January, 1660 (1659 A.S.). There was no Royal Governorship at the time of the "Protectorate", and the Governor technically answered to the Cromwellian Parliament, although Royalist sentiment was prevalent in the colony of Virginia at this time. The former Royalist governor Berkeley arrived to replace him March 13, 1660.
Samuel Mathews (Jr.) was the elder son of Samuel Matthews (Sr.) (1572-1657) and Frances Grevill West Peirsey Mathews (1590-1635). He was born at his father's plantation, Mathews Manor, later known as Denbigh, which was located on the north side of the James River at Blunt Point, the confluence of the Warwick and the James rivers in the area which later became Warwick County, Virginia (and which is now within the city limits of Newport News).
The elder Samuel Mathews was the first of the Mathews family to emigrate from England to Virginia, arriving at Jamestown by 1619. He eventually had several other land holdings, including one near Henricus and another at Old Point Comfort. Known as Colonel Mathews, the elder Samuel became one of the most prominent men in the colony. He was a member of the Governor's Council and was actively involved in conflicts with the Native Americans. In 1635, he was one of the leaders of the popular mutiny that ousted Royal Governor Sir John Harvey. Upon returning to England, the elder Mathews was eventually cleared of any charges; upon returning to Virginia, he resumed service on the Governor's Council until 1644.