Rancho San Gregorio was a 17,783-acre (71.97 km2) Mexican land grant in present day San Mateo County, California given in 1839 by Governor Juan Alvarado to Antonio Buelna. At the time, the grant was in Santa Cruz County; an 1868 boundary adjustment gave the land to San Mateo County. The rancho extended from Tunitas Creek in the north to the mouth of Pomponio Creek and encompassed San Gregorio, California, San Gregorio State Beach and La Honda
Antonio Jose Buelna (1790–1846), son of José Antonio Buelna (1754–1821) married Maria Concepción Valencia (b.1798) in 1816. In 1836, José Castro, Juan Alvarado, Antonio Buelna, and José Antonio de la Guerra (son José de la Guerra y Noriega) signed the demand that Governor Nicolás Gutiérrez resign. In addition to the four square league Rancho San Gregorio grant, Alvarado granted Antonio Buelna Rancho San Francisquito in Santa Clara County in 1839. Buelna built a road, known today as La Honda Road and Old La Honda Road, over the hills connecting his two ranchos.
Buelna in 1842, made a will by which he left to his wife, Maria Concepción Valencia, Juan Bautista Buelna, and three others, each an undivided one-fifth share of the rancho. After Antonio Buelna died in 1846, María Concepción Valencia married Francisco Rodriguez, a widower and grantee of Rancho Arroyo del Rodeo. In 1849, María Concepción Valencia de Rodríguez sold a one square league of the eastern portion of Rancho San Gregorio to Salvador Castro.
With the cession of California to the United States following the Mexican-American War, the 1848 Treaty of Guadalupe Hidalgo provided that the land grants would be honored. Separate claims for Rancho San Gregorio, required by the Land Act of 1851, were presented by the widow of Buelna and Salvador Castro for their respective portions of Rancho San Gregorio.