Rancho San Dieguito was a 8,824-acre (35.71 km2) Mexican land grant in present day San Diego County, California given in 1845 by Governor Pío Pico to Juan María Osuna. The rancho property was renamed Rancho Santa Fe in 1922 by the Santa Fe Land Company.
Juan Maria Osuna, son of one of the "leather-jacket soldiers", also became a soldier and for many years served at the Presidio of San Diego. Osuna played an important role in the establishment of the pueblo of San Diego (population 150) and was elected alcalde. In addition, Juan Osuna became a Justice of the Peace and was administrator of the San Diego Mission. As Mayor, one of Juan Maria Osuna's powers included the approval of petitions for land and he quiet naturally approved his own application for the San Dieguito grant. In 1845, Governor Pio Pico awarded him full title to the two square league Rancho San Dieguito.
In 1806, Osuna married Maria Juliana Josepha Lopez (1791-1871). Osuna built an adobe home on the ranch for himself and his wife, and gave an existing adobe to his son, Leandro. Osuna was considered a fine alcalde but was also a gambling man and lost some of his land to pay off bad debts. Leandro Osuna took possession of the ranch in 1851 when his father died. A veteran of the Battle of San Pasqual in 1846, Leandro died in 1859; he was thirty-seven years old. The care of Rancho San Diequito fell to Juan Osuna's widow, Juliana Lopez de Osuna.
With the cession of California to the United States following the Mexican-American War, the 1848 Treaty of Guadalupe Hidalgo provided that the land grants would be honored. As required by the Land Act of 1851, a claim for Rancho San Dieguito was filed with the Public Land Commission in 1852, and the grant was patented to Juliana Lopez de Osuna in 1871.