Ponchatoula Creek is a 26.1-mile-long (42.0 km) tributary of the Natalbany River in Tangipahoa Parish, Louisiana. The two waterways join where a section of the Natalbany forms the boundary between Tangipahoa Parish and Livingston Parish. Ponchatoula Creek originates west of Old US Highway 51, 1 mile (1.6 km) north of Independence. The creek is entirely within Tangipahoa Parish.
Ponchatoula Creek's cardinal direction is southwest (or south and west) as it meanders south around the west side of Independence and Tickfaw, and even southeast through Natalbany and the northeastern quadrant of Hammond, thence westward between Hammond and the city of Ponchatoula, and then south and west to join the Natalbany River between Springfield and Lake Maurepas.
Ponchatoula Creek is bridged by LA 40, LA 1063, LA 442, US 51, LA 1064 (Natalbany Road), North Oak Street, Canadian National Railway, LA 3234 (University Avenue), LA 1065 (North Cherry Street), US 190, US 51 Business, I-55 (in its concurrency with US 51), and LA 22 before emptying into the Natalbany River southwest of Ponchatoula. One tributary forms part of the northern perimeter of Southeastern Louisiana University's main campus, and another tributary flows under the campus in a tunnel starting on the south side of LA 3234 near the Southeastern University Center.