Nathaniel Giles (1558–1633 or 1634) was an English Renaissance organist and composer. He was the organist for Worcester Cathedral and wrote Anglican anthems. While Master of the Children of the Chapel Royal he took over Blackfriars Theatre in a business arrangement with producer Henry Evans and there he worked with Ben Jonson on a children's company. He was also a master of the Choir of St George's Chapel, Windsor Castle and an organist at Westminster Abbey.
His Te Deum (Durham Cathedral manuscript A2, folio 56) has instructions for the organist to play an octave lower than written, and one assumes this is to give the 'dark' sonority of a 10 ft organ stop rather than the everyday colour of the standard 5 ft Principal stop of the Tudor organ. His Vestigia mea dirige was included in the manuscript collection known as the Dow Partbooks.
His son, Nathaniel Giles became a Canon of Windsor.