Listán negro (also known as Listan Prieto) is a red Spanish wine grape variety that is widely planted in the Canary Islands, particularly on the island of Tenerife where it is a permitted variety in the Denominaciones de Origen (DO) wines of Tacoronte-Acentejo, Valle de la Orotava, Ycoden-Daute-Isora and Valle de Güímar. It is also permitted in the Spanish wine regions of El Hierro, Gran Canaria, La Gomera, La Palma, Lanzarote. Across the Canary Islands more than 5,000 hectares (12,000 acres) are planted to the variety. Listán negro is the black-skin version of the Palomino grape (Listan Blanco) that is used in the production of the fortified wine Sherry.
In 2007, DNA fingerprinting done by the Centro Nacional de Biotecnología in Madrid, Spain discovered that the Mission grape that was widely planted in the earliest New World vineyards in the America was a genetic match to Listán negro. Despite the genetic match, there is enough clonal variation that has occurred over the centuries of geographical separation that the Mission grape of the Americas and the Listán negro grape of the Canary Islands are classified by the Vitis International Variety Catalogue as two separate grape varieties. Part of the variation is likely because some of earliest plantings by the Spanish missionaries were from grape seeds which are the result of pollination and sexual propagation and thus more likely to have slight differences from the parent vine than propagation through cuttings.