Mikulovská is one of four Czech wine regions within southern Moravia, Czech Republic. The Mikulovská wine region, containing 30 wine villages, is famed for producing white wines grown in the limestone soils, particularly in and around the area of the Pálava Landscape Protected Area. Historically a centre of viticulture and winemaking in Moravia, Mikulovská is home to the historical town of Mikulov, and the Czech National Wine Centre (Czech: Národní Vinarské Centrum) and Wine Salon of the Czech Republic, located at Valtice Castle.
In the 2nd Century CE, the Roman 10th Legion based at Vindobona built an extensive outpost near the Amber Road and the Pálava Hills in Mikulovská, near the present-day village of Pasohlávky. Around the year 278, the Roman Emperor Marcus Aurelius Probus annulled the edict of Emperor Domitian that had prohibited the planting of grapes in colonies north of the Alps, and encouraged the planting of new vines in the northern Roman colonies. Modern-day archaeological excavations of the ancient Roman outpost near Pasohlávky have yielded many artifacts, including a vine pruning knife. Wine historians theorize that, during the Roman occupation, the Grüner Veltliner and Welschriesling grape varieties may have been introduced to the region. Viticulture was practiced during the Great Moravian Empire (833–906), as evidenced by numerous pruning knives and grape seeds unearthed during archaeological excavations of Slavic settlements.