Mayo is a village in Yukon, Canada, along the Silver Trail and the Stewart River. The population at the 2011 census was 226, a decrease of 8.9% from the 2006 census. It is also the home of the First Nation of Nacho Nyak Dun, whose primary language is Northern Tutchone. Nacho Nyak Dun translates into "big river people". It is serviced by Mayo Airport. It was formerly known as Mayo Landing.
The only school is J. V. Clark School, which is named after Dr. Clark. It had about 70 students in 2012. The current Principal is Ken MacGillivray.
The village was named after former circus acrobat turned settler and explorer Alfred Mayo.
Before Europeans came there were in the area two communities of the Nacho Nyak Dun people, who lived by hunting and trapping. The river now known as the Stewart River was known as the "Náhcho Nyäk" ("Great River"). The people lived across the Stewart River from the main focus of today's Mayo, in a district today called "Old Mayo village". The old settlement was reinstated on the initiative of a missionary, but in 1934 the river burst its banks and flattened much of the old village, destroying the church and many cultural treasures.
The first gold discoveries in the area were made in the 1880s: silver was also discovered some time later. Till the mid-twentieth century Mayo was connected with the outside world by the river and received any supplies by boat. In the 1950s the construction of the Klondike Highway and the Silver Trail provided Mayo with a road link to Stewart Crossing.
Between 1973 and 1984 negotiation took place between the government and the northern leaders over land rights and self-government. A breakthrough came only in 1993 with a treaty between the residents and the lawmakers concerning an area of 1,830 sq mi (4,700 km2) and a payment, over fifteen years, totalling C$14.5 million.