Princess Tutu was originally broadcast in two seasons. The first season, "Kapitel des Eies" ("Chapter of the Egg"), consisted of 13 half-hour episodes. The second season was broadcast as 24 quarter-hour episodes and one half hour episode, to conform to the format of the time slot, so that each episode was split into two parts. These were brought back together in the DVD release as 13 complete episodes. The second season is called "Kapitel des Jungens" ("Chapter of the Chick") in R2 DVDs, and "Kapitel (sic) des Kükens" ("Chapter of the Fledgling") in R1 DVDs.
In 2004 ADV Films announced that they had licensed the anime series for distribution in North America. ADV Films produced English adaptations for all episodes and, beginning in 2005, the series was periodically released as single DVD "volumes" that each contained several episodes. In 2007 the series was released as a complete DVD collection of all 26 episodes. In 2011 AEsir Holdings announced the licensing of the series and the release of a complete DVD collection of all 26 episodes distributed by Section23 Films.
Ahiru awakes from a dream in which she is a duck watching a handsome prince dance ballet. Later, she walks early into class and discovers Mytho, a student in the advanced class, dancing alone. She has a nerve-racking conversation with him before Fakir, another student, walks in. Fakir is revealed to be domineering and condescending towards Mytho. In ballet class, we learn that Mytho is dating a girl named Rue. The teacher is a cat, yet Ahiru is the only one in the class to find this strange.
Rue is revealed to be a talented girl in the advanced class, who most of the students look up to. After class, Ahiru goes to the boy’s dorm to apologize to Mytho for accidentally causing him to injure his leg. Before she can work up the courage to go in, Fakir stops her and turns her away.
Later that night, Ahiru regrets not being able to apologize to Mytho, and ponders his lonely eyes. She wishes to help him, catching the attention of the mysterious Drosselmeyer, who says to himself: “As the water flows, time has begun to move. Now, let me listen to your story!”
The next day, Duck wakes up to realize that she is just a duck. Drosselmeyer appears to tell her that she is also the legendary Princess Tutu; her task is to return the pieces of heart to the Prince, Mytho. Longing to one day see Mytho smile, she decides to take up the challenge. Drosselmeyer gives back the pendant that changes her into a girl, as well as Princess Tutu.