"Let's Do It Again" was a hit song for The Staple Singers. Written by Curtis Mayfield, it was part of the soundtrack for the Bill Cosby/Sidney Poitier movie, Let's Do It Again. The single reached the top of the Billboard Hot 100 singles chart on December 27, 1975, the day before Roebuck "Pops" Staples' 61st birthday, and also spent two non-consecutive weeks at the top of the Hot Soul Singles chart. It was the last major hit by the group.

The song was covered by George Benson for his 1988 album, Twice the Love, and was also released as a single. R&B quartet Xscape sampled the song on its first single, "Just Kickin' It," from the 1993 album Hummin' Comin' at 'Cha and four years later, the group sampled the song of the same name on the final chorus before the end of the song from the soundtrack to the film, Soul Food, which was originally produced by Jermaine Dupri. Ice Cube sampled the song in the remix of "It Was a Good Day". It was also sampled in John Legend's song "Number One".