Karin Göransdotter Gyllenstierna (floruit 1530s - 1602) was a Swedish courtier. She served as hovmästarinna (senior lady in waiting, or mistress of the Robes) to queen Catherine Jagellon and, after her death, to Princess Anna Vasa of Sweden.
Karin was the daughter of riksråd Göran Eriksson Gyllenstierna of Fogelvik (- 1575) and Kerstin Nilsdotter Grip (- 1538). She was thereby a relative of the famous Christina Gyllenstierna. She married Nils Månsson Natt och Dag in 1553. She was thereby the sister-in-law of Margareta Birgersdotter Grip, who were also her neighbor on the estate Bro. She became a widow in 1554, and gave birth to her son Nils Nilsson Natt och Dag (1554-1613) posthumously. As the guardian of her son, she managed his estate until he was declared of legal majority in 1573.
In 1566, she became engaged to riksråd Ivar Ivarsson Liljeörn. She never married him, but the couple consummated their engagement and had a son, Ivar Ivarsson (1567-1590). In 1567, her fiancé was murdered during the Sture Murders. Despite never being married to Liljeörn, she took control of his property, considering it the inheritance of herself and her son after her betrothed. This entangled her in a lawsuit over the inheritance with his relatives, which lasted for the rest of her life. In 1587, the King declared his support for her right in the dispute. This did not end it, however. In 1594, her rights were for a second time confirmed over that of the relatives of her late fiancé, but again without the feud being put to an end.
Karin Gyllenstierna was given the position of senior lady in waiting and Mistress of the Robes, or hovmastarinna (Court Mistress), to queen Catherine Jagellon. After the death of the queen in 1583, she was given the equivalent position at the court of Princess Anna Vasa of Sweden. She is mentioned in this position in 1587, when the next Mistress of the Robes to the (new) queen of Sweden is also recorded.