John Jonston (in Polish, Jan Jonston; in Latin, Joannes Jonstonus; Szamotuły, 15 September 1603 – 1675, Legnica) was a Polish scholar and physician, descended from Scottish nobility and closely associated with the Polish magnate family of the Leszczyńskis.
Jonston was born in Szamotuły, the son of Simon Johnston, who had emigrated to the Polish-Lithuanian Commonwealth from Scotland. Jonston's early education was sponsored by one of his two paternal uncles who had come to the Commonwealth with the father.
From 1611 Jonston attended the school of the Bohemian Brothers in Ostroróg, then the Schoenaichianum in Bytom, and from 1619 the gymnasium in Toruń, Royal Prussia. As a Calvinist, he could not attend the Catholic-controlled Jagiellonian University. Consequently he earned his first degree at the University of St Andrews (1622–25; M.A., 1623), where he studied theology, scholastic philosophy, and the Hebrew language; his sponsors included the Primate of All Scotland, John Spottiswood.