John I of Brienne (died 12 June 1294) was the son of Alphonso of Brienne and Marie de Lusigan. His mother was the heiress of Eu, Seine-Maritime, and he succeeded his father as Count of Eu in 1270.
He married Beatrice, the daughter of Guy III, Count of Saint-Pol. They had five children:
John died at Clermont-en-Beauvaisis in 1294.