John Clotworthy, 1st Viscount Massereene (died September 1665) was a prominent Anglo-Irish politician.
He was the son and heir of Sir Hugh Clotworthy (died 1630), High Sheriff of Antrim (who first came to Ireland as agent of the Irish Society in connection with the colonisation of Londonderry), by his wife Mary Langford, daughter of Roger Langford of West Downe in the parish of Broadwoodwidger in Devon. A sculpted escutcheon showing the arms of Clotworthy impaling Langford of Kilmackedret was displayed on the facade of Antrim Castle, now demolished. Sir Hugh Clotworthy was the second son of Thomas Clotworthy (born 1530) of Clotworthy in the parish of Wembworthy in Devon, by his third wife Dorothy Parker, a daughter of John Parker (ancestor of the Earl of Morley(1815)) of North Molton in Devon. Sir Hugh's paternal grandmother was Ivota/Abbot Rashleigh, heiress of Rashleigh in Wembworthy, Devon, to which seat at some time before 1640 the senior line of the Clotworthy family eventually moved their residence from the nearby ancestral seat of Clotworthy.
John was elected to the Irish House of Commons as member for County Antrim in 1634, and was a member both of the Short and of the Long Parliament in England, in 1640, representing Bossiney in Cornwall. Clotworthy was a vehement opponent of Thomas Wentworth, 1st Earl of Strafford, in whose impeachment he took an active share. He also took part in the prosecution of Archbishop Laud. He seems to have felt a deep personal hatred for both Strafford and Laud, springing perhaps from profound religious differences. He was criticised for his conduct at Laud's execution, where he thrust himself forward and harangued that elderly man, who was trying to prepare himself for death, on his alleged religious errors