Johann Pistorius (January 1504, Nidda, Hesse – 25 January 1583, Nidda) was a German Protestant minister. From 1541 he was the Superintendent at the church in Nidda in Hesse.
Along with Philipp Melanchthon and Martin Bucer, Pistorius was appointed by Charles V to represent the Protestants at the Diet of Regensburg in 1541. He also participated in the Colloquy of Worms in 1557.
Pistorius was the father of Johann Pistorius (the Younger).