J. B. Hughes (John Bristow Hughes; July 1817 – 25 March 1881) was a grazier, developer and politician in the early days of the Colony of South Australia.
Born in Kentish Town, London, in July. 1817, he was employed at the age of 13 in the office of a merchant who sent him to Calcutta where his health was badly affected. John and his brother Herbert Bristow Hughes came to South Australia in 1840 by way of Tasmania. By August 1841 he was running a sheep-station on the River Gilbert with between 3,000 and 5,000 sheep. He expanded northwards, then sold his run at a substantial profit. His next venture was into real estate, with housing in Woodville; successful enough to donate land for the Anglican Church. Perhaps he also made a substantial contribution to the building.
He was heavily involved around 1849 in the foundation of St. Peter's College, a cause that was close to his heart the rest of his life - the "Letters" page of the South Australian Register frequently bore his missives complaining of mis-management or lack of vision.
He stood successfully in 1855 for the seat of East Torrens in the original Legislative Council (when one-third of members was nominated by the South Australian Company) and he took part in the framing the Constitution, then in South Australian colonial election, 1857 stood successfully for the House of Assembly seat of Port Adelaide. He supported Torrens Title and served as Treasurer of South Australia from 1 September to 30 September 1857, and was complimented for his Budget speech. On 24 September 1858 be resigned his seat to visit England, in the hope of a recovery from his chronic health complaints but he did later stand (unsuccessfully) for a seat in the House of Assembly. He supported independence of Church and State, opposed State aid to non-government schools, and was described as "anti-Ritualist".