Sir Humfrey Tufton, 1st Baronet (1584 – October 1659) was an English politician who sat in the House of Commons from 1640 to 1648.
Tufton was the son of Sir John Tufton, 1st Baronet of Hothfield and brother of Nicholas Tufton, 1st Earl of Thanet. He purchased The Mote near Maidstone.
In November 1640, Tufton was elected Member of Parliament for Maidstone in the Long Parliament. He was created a baronet, of The Mote in the County of Kent, on 24 December 1641. He was excluded from parliament under Pride's Purge in 1648. He was appointed High Sheriff of Kent for 1654.
Tufton died at Bobbing Place at the age of 76.
Tufton married Margaret Morley, daughter of Herbert Morley of Glynde Sussex. He was succeeded in the baronetcy by his son John. The title became extinct on the death of the second Baronet in 1685. His daughter Olympia married Sir William Wray, 1st Baronet, of Ashby.