Stratification in the field of ecology refers to the vertical layering of a habitat; the arrangement of vegetation in layers. It classifies the layers (sing. stratum, pl. strata) of vegetation largely according to the different heights to which their plants grow. The individual layers are inhabited by different animal and plant communities (stratozones).
The following layers are generally distinguished: forest floor (root and soil layers), herbaceous, shrub, understory and canopy layers. These vegetation layers are primarily determined by the height of their individual plants, the different elements may however have a range of heights. The actual layer is characterised by the height range in which the vast majority of photosynthetic organs (predominantly leaves) are found. Taller species will have part of their shoot system in the underlying layers. In addition to the above-ground stratification there is also a “root layer”. In the broadest sense, the layering of diaspores in the soil may be counted as part of the vertical structure. The plants of a layer, especially with regard to their way of life and correspondingly similar root distribution interact closely and compete strongly for space, light, water and nutrients. The stratification of a plant community is the result of long selection and adaptation processes. Through the formation of different layers a given habitat is better utilized. Strongly vertically stratified habitats are very stable ecosystems. The opposite is not true, because several less stratified vegetation types, such as reed beds, can be very stable. The layers of a habitat are closely interrelated and at least partly interdependent. This is often the case as a result of the changes in microclimate of the top layers, the light factor being of particular importance.