Henry Barrowe (or Barrow) (c. 1550 – 6 April 1593) was an English Separatist Puritan, executed for his views.
Barrowe was born about 1550, in Norfolk, of a family related by marriage to Nicholas Bacon, and probably to John Aylmer, Bishop of London. He matriculated at Clare Hall, Cambridge, in November 1566, and graduated B.A. in 1569–1570. Afterwards he "followed the court" for some time, leading a frivolous if not licentious life. He was a member of Gray's Inn for a few years from 1576, but was never called to the bar.
In about 1580 or 1581 Barrowe was impressed by a sermon; he retired to the country, and was led by study and meditation to a strict form of Puritanism. Subsequently he came into close relations with John Greenwood, the Separatist leader, whose views he adopted. He was associated with "the brethren of the Separation" in London and their secret meetings.
Greenwood was imprisoned in The Clink, and Barrowe came from the country to visit him. On 19 November 1586 he was detained by the gaoler and brought before Archbishop John Whitgift. He insisted on the illegality of this arrest, refused either to take the ex officio oath or to give bail for future appearance, and was committed to the Gatehouse Prison. After nearly six months detention and several irregular examinations before the high commissioners, he and Greenwood were formally indicted (May 1587) for recusancy under an act originally directed against Roman Catholics. They were ordered to find heavy bail for conformity, and to remain in the Fleet Prison until it was forthcoming.