Henry of Anhalt-Köthen (30 July 1778, Schloss Pless – 23 November 1847, Köthen) was a German prince of the House of Ascania, ruler of the non-sovereign principality of Anhalt-Pless and the last ruler of the duchy of Anhalt-Köthen.
He was the fourth (but third surviving) son of Frederick Erdmann, Prince of Anhalt-Pless, by his wife, Louise Ferdinande, daughter of Henry Ernest, Count of Stolberg-Wernigerode.
In 1796, he joined the Prussian army. During the campaign of 1806, he attained the rank of major. Before his retirement from active service, Henry was elevated to the rank of Generalmajor.
After his elder brother Frederick Ferdinand inherited Anhalt-Köthen in 1818, Henry assumed the government over the state country of Pless. When Frederick Ferdinand died in 1830, Henry succeeded him in Köthen, whereas he left Pless to his younger brother Louis, who died in 1841 without heirs. Henry reassumed his rulership over Pless until his death.
In Trebschen on 18 May 1819 Henry married Auguste Fredericka Espérance (3 August 1794, Brunswick – 13 July 1855, Köthen), daughter of Henry XLIV, Prince Reuss of Köstritz, and sister of Prince Henry LXIII. The union was childless.