The Greek Cup 2000–01 was the 59th edition of The Greek Football Cup, or Greek Cup for short.
Totally 50 teams participated, 16 from Alpha Ethniki, 16 from Beta, and 18 from Gamma. It was held in 6 rounds, included final. An Additional Round was held between First and Second, with 2 matches, in order that the teams would continue to be 16. In the First round, there were 8 groups with 6 teams each, while 2 teams qualified without matches in that phase.
For first time were established double matches in the phase of groups, thus each team played 10 matches. Because the phase began very early, in a period that teams should play preparation friendlies, the Hellenic Football Federation (EPO) allowed at the 5 first matches a maximum of 7 substitutions, something unusual in Greece, very probably and internationally, for matches of an official competition.
There were very interesting confrontations, after drawing elected "strong" pairs. In the Second round (phase of 16) Olympiacos eliminated AEK Athens with two wins. The first match in Nikos Goumas Stadium awarded to Olympiacos 2–0 in cards, in an eventful match that was abandoned against Enosis and while the score was 1–1. In the second match, at Athens Olympic Stadium, Olympiacos home then, they shattered AEK Athens 6–1. In the same round, in Thessaloniki's derby between PAOK and Aris, the first qualified with a 1–1 draw and a 2–0 victory in second match. Remarkable in this phase was also the qualification of Panathinaikos against Panionios with score 7–2, while the first match between two teams had been ended 1–0 for Panionios.
In quarter-finals followed the conflict between Olympiacos and Panathinaikos. Afterwards undecided 1–1 in Athens Olympic Stadium, the reds triumphed 4–1 in Apostolos Nikolaidis Stadium against their opponents. More shocking was the semi-final between Olympiacos and Iraklis. Olympiacos with two victories, 1–0 away and 5–4 in home, in an impressive match where 10 minutes before the end the score was 3–4, qualified in the Final.