Giulio Spisanelli (died 1658) was an Italian painter of the Baroque period, active mainly in Bologna.
Spisanelli was born at Bologna. His brother was the painter Ippolito Bolognese. He first trained under the painter Vincenzo Spisanelli, his father, then under Domenico Maria Canuti. He traveled to Rome at the instigation of his father, but died soon after returning to Bologna.