Giulia della Rovere (1531, Casteldurante – 4 April 1563, Ferrara) was an Italian noblewoman. A portrait of her by Titian survives in the Palazzo Pitti in Florence.
She was the daughter of Francesco Maria I Della Rovere and Eleonora Gonzaga. On 3 January 1549 she married Alfonso d'Este, an illegitimate son of Alfonso I d'Este, duke of Ferrara, and of Laura Dianti. They had three children: