Giovanni Piccolomini (1475–1537) was an Italian papal legate and Cardinal. He was a nephew of Pope Pius III.
He was archbishop of Siena in 1503, bishop of Sitten 1522, bishop of Aquila 1523, bishop of Albano in 1524, bishop of Palestrina in 1531, bishop of Porto e Santa Rufina in 1533, bishop of Ostia in 1535. Dean of the College of Cardinals in October 1534.