Gianfrancesco Gambara (1533–1587) was an Italian Roman Catholic cardinal and bishop.
Gianfrancesco Gambara was born in Brescia on February 16, 1533, the son of Brunoro Gambara, count of Pralboino (a field marshal in the army of Charles V, Holy Roman Emperor) and Virginia Pallavicini, widow of Ranuccio Farnese. He was the nephew of Cardinal Uberto Gambara. After his father's death, his mother married a third time, to Gilberto Borromeo, making Charles Borromeo his stepbrother.
He began his studies at the University of Padua, then continued at the University of Bologna. He completed his studies at the University of Perugia, becoming a doctor of both laws.
He then joined his uncle the cardinal at the court of Charles V. He later traveled to Rome and joined the court of Pope Julius III, who made him a privy chamberlain. During the pontificate of Pope Pius IV, he became a cleric of the Apostolic Camera, eventually becoming its president.
Pope Pius IV made him a cardinal deacon in the consistory of February 26, 1561. He received the red hat and the titular church of Santi Marcellino e Pietro al Laterano (declared a deaconry pro illa vice) on March 10, 1561.