Florida State Road 329
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| Route information | ||||
| Maintained by FDOT | ||||
| Major junctions | ||||
| South end: |
   SR 20 / SR 24 / SR 26 in Gainesville
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| North end: |
 SR 20 / CR 329 in Gainesville |
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| Location | ||||
| Counties: | Alachua | |||
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State Road 329 (SR 329) is a short state highway traveling south and north through Gainesville, Florida. It is named North Main Street. The road is concurrent with SR 20 for its entire length. South Main Street in Gainesville was the southern portion of SR 329 before FDOT relinquished it to the city.
SR 329 starts at an intersection with SR 20/SR 24/SR 26. It ends at an intersection with CR 329 and Northwest Eighth Avenue, where SR 20 turns west.
FDOT and Alachua County made an agreement for transfer of South Main Street in March 2005, but did not consummate the transfer until April 2012 (north of Depot Avenue) and June 2013 (south of Depot Avenue). Subsequently, in August 2013, the county transferred the portion north of Southwest 16th Avenue (SR 24A/SR 226) to the city of Gainesville.
The entire route is in Gainesville, Alachua County.
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