Florida State Road 121
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| Route information | ||||
| Maintained by FDOT | ||||
| Length: | 101.867 mi (163.939 km) | |||
| Major junctions | ||||
| South end: |
  US 19 / US 98 at Lebanon Station
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  US 27 / US 41 in Williston I-75 in Gainesville I-10 in Macclenny
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| North end: |
  SR 23 / SR 121 near Stokesville, GA
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| Location: | LaCrosse |
State Road 121 (SR 121) is a major state highway that runs north and south in northern Florida. The road is part of a long multi-state route that also goes through Georgia and South Carolina.
Beginning in Lebanon Station at a channelized intersection with US 19-98 and County Road 336, State Road 121 runs through Goethe State Forest where it turns east and intersects with CR 337. The road makes a sharp left curve to the northeast where it runs through desolate farmland; the only resemblance to a major intersection is CR 326. Within the city of Williston, SR 121 overlaps CR 316, until the intersection with US 41. It then joins US 41 as CR 316 continues eastward, which then joins the east end of ALT US 27, and then the US 27-41 concurrency before breaking away to the northeast again, taking the DeSoto Trail away from US 41.
North of CR 335, SR 121 hugs the west side of the Levy-Marion County Line, but never crosses it, choosing to enter Alachua County instead. Immediately after the interchange with Interstate 75(Exit 382), SR 121 makes a sharp left onto Northwest 34th Street as SR 331 becomes the designation for Williston Road. As it crosses SR 24 it borders the University of Florida to the east until crossing SR 26A and then SR 26. North of US 441, the DeSoto Trail breaks away.
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