Cross matching

Cross-matching
Compatibility testing concerning RBCs
MeSH	D001788
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In transfusion medicine, cross-matching or crossmatching (sometimes called compatibility testing) is testing before a blood transfusion to determine if the donor's blood is with the blood of an intended recipient. Cross-matching is also used to determine compatibility between a donor and recipient in organ transplantation. Compatibility is determined through matching of different blood group systems, the most important of which are the ABO and Rh system, and/or by directly testing for the presence of antibodies against the antigens in a sample of donor blood or other tissue.

Cross-matching is done by a certified laboratory technologist in a medical laboratory. It can be done electronically with a database if a patient has previously been tested, or serologically by physical testing if not. Simpler tests may be used to determine blood type (only), or to screen for antibodies (only) (indirect Coombs test).

Immediate-spin cross-matching is an abbreviated form of cross-matching that is faster, less expensive but also less sensitive. It is an immediate test that combines the patient's serum and donor's red blood cells at room temperature. No agglutination indicates a negative test reaction, or compatible match. Indications for ISCM are dependent on the circumstances of the patient and it can be used in place of a full cross-match or performed as a preliminary test.

Electronic cross-matching is a computer-assisted analysis using data, from the donor unit (where a donor's blood is tested prior to donation) and testing done on blood samples from the intended recipient. This includes ABO/Rh typing of the unit and of the recipient, and an antibody screen of the recipient. Electronic cross-matching can only be used if a patient has a negative antibody screen, which means that they do not have any active red blood cell atypical antibodies, or they are below the detectable level of current testing methods. If all of the data entered is compatible, the computer will print a compatibility label stating that the unit is safe to transfuse.